PFUPISO UYE TSANANGURO MUYEdzo
Leptospirosis inoitika pasi rose uye idambudziko rakajairika kusvika kune rakasimba rehutano kuvanhu nemhuka, kunyanya munzvimbo dzine mamiriro ekunze anopisa uye ane hunyoro.Matura echisikigo e leptospirosis makonzo pamwe nemhando huru yemhuka dzinoyamwisa.Utachiona hwevanhu hunokonzerwa neL. interrogans, nhengo yepathogenic yegenus yeLeptospira.Chirwere ichi chinotapurirwa neweti kubva kumhuka inobatwa.
Mushure mekutapukirwa, maleptospires anowanikwa muropa kusvikira acheneswa mushure memazuva mana kusvika ku7 zvichitevera kugadzirwa kwe anti-L.interrogans antibodies, yekutanga yekirasi yeIgM.Tsika yeropa, weti uye cerebrospinal fluid inzira inoshanda yekusimbisa kuongororwa mukati memavhiki e1 kusvika ku2 mushure mekusangana.Serological kuonekwa kweAntiL.interrogans masoja ekudzivirira chirwere inzira yakajairika yekuongorora.Miedzo inowanikwa pasi pechikamu ichi: 1) The microscopic agglutination test (MAT);2) ELISA;3) Indirect fluorescent antibody bvunzo (IFATs).Nekudaro, nzira dzese dzataurwa pamusoro dzinoda nzvimbo yakasarudzika uye matekinoroji akadzidziswa.
Leptospira IgG/IgM iri nyore serological bvunzo inoshandisa maantigens kubva kuL. interrogans uye inoona masoja ekudzivirira chirwere eIgG neIgM kumatumbu aya panguva imwe chete.Muedzo unogona kuitwa nevashandi vasina kudzidziswa kana hunyanzvi, vasina midziyo yerabhoritari inorema uye mhedzisiro inowanikwa mukati memaminitsi gumi nemashanu.
PRINCIPLE
Iyo Leptospira IgG/IgM Rapid Test Kit ndeye lateral kuyerera chromatographic.
immunoassay.The test cassette ine: 1) burgundy colored conjugate pad ine recombinant L. interrogans antigens conjugated ne colloid gold (Leptospira conjugates) uye tsuro IgG-goridhe conjugates, 2) nitrocellulose membrane strip ine maviri ebvunzo mabhendi (M uye G mabhendi) uye bhandi rekutonga (C band).Iyo M band yakagara yakavharwa nemonoclonal anti-human IgM yekuona anti-L.. interrogans IgM, G bhendi rakagara rakaputirwa nemareagents ekuona anti-L.interrogans IgG, uye C bhendi rakafanoputirwa nembudzi anti tsuro IgG.
Kana vhoriyamu yakakwana yemuenzaniso webvunzo yaiswa mumuenzaniso tsime rekaseti, mufananidzo wacho unotama necapillary chiito kuyambuka kaseti.IgM anti-L.. interrogans kana iripo mumuenzaniso ichasunga kune Leptospira conjugates.Iyo immunocomplex inobva yatorwa pa membrane ne pre-coated anti-human IgM antibody, ichiita burgundy colored M band, zvichiratidza L.. interrogans IgM positive test result.IgG anti-L.. interrogans kana iripo mumuenzaniso ichasunga kune Leptospira conjugates.Iyo immunocomplex inobva yatorwa ne-pre-coated reagents pa membrane, ichiita burgundy colored G band, zvichiratidza L.. interrogans IgG positive test result.
Kusavapo kwechero mabhendi ebvunzo (M naG) kunoratidza mhedzisiro yakaipa.Iyo bvunzo ine yemukati kudzora (C bhendi) iyo inofanirwa kuratidza burgundy ruvara bhendi ye immunocomplex yembudzi anti rabbit IgG/tsuro IgG-goridhe conjugate zvisinei nekusimudzirwa kwemavara pane chero mabhendi ebvunzo.Zvikasadaro, mhedzisiro yebvunzo haishande uye mufananidzo unofanirwa kuongororwa nemumwe mudziyo.
