PFUPISO UYE TSANANGURO MUYEdzo
Malaria chirwere chinotakurwa neumhutu, hemolytic, febrile chinobata vanhu vanopfuura mamirioni mazana maviri uye chinouraya vanhu vanopfuura miriyoni imwe pagore.Inokonzerwa nemhando ina dzePlasmodium: P. falciparum, P. vivax, P. ovale, uye P. malariae.Iyi plasmodia yose inotapukira uye inoparadza erythrocytes yevanhu, ichibudisa chills, fever, anemia, uye splenomegaly.P. falciparum inokonzeresa zvirwere zvakanyanya kudarika mamwe marudzi eplasmodial uye inokonzeresa ndufu zhinji dzemalaria.P. falciparum neP. vivax ndiwo utachiona hunozivikanwa zvikuru, zvisinei, pane musiyano mukuru wenzvimbo mukuparadzirwa kwemarudzi.
Sechinyakare, marariya inoonekwa nekuratidzwa kwezvipenyu paGiemsa yakasvibiswa gobvu smears yeropa rekutenderera, uye marudzi akasiyana eplasmodium anosiyaniswa nekuonekwa kwavo mune hutachiona erythrocytes.Iyo tekinoroji inokwanisa kunyatso uye yakavimbika kuongororwa, asi chete kana ichiitwa nehunyanzvi maikorosikopu vachishandisa yakatsanangurwa maprotocol, ayo anopa zvipingamupinyi zvikuru kunzvimbo dziri kure nedzirombo dzepasirese.
Iyo Malaria Pf/Pv Ag Rapid Test inogadzirwa kugadzirisa zvipingaidzo izvi.Inoshandisa masoja ekudzivirira chirwere akananga kuP. falciparum Histidine Rich Protein-II (pHRP-II) uye kuP. vivax Lactate Dehydrogenase (Pv-LDH) panguva imwe chete kuona uye kusiyanisa utachiona neP. falciparum uye P. vivax.Muedzo unogona kuitwa nevashandi vasina kudzidziswa kana hunyanzvi hushoma, vasina michina yemurabhoritari
PRINCIPLE
Iyo Malaria Pf/Pv Ag Rapid Test is a lateral flow chromatographic immunoassay.Iwo mitsetse yekuyedza zvikamu zvinosanganisira: 1) burgundy yeruvara conjugate pad ine mbeva inopesana nePv-LDH antibody yakasanganiswa negoridhe colloid (Pv-LDH-goridhe conjugates) uye mbeva anti-pHRP-II antibody yakasanganiswa negoridhe colloid (pHRP-II). -gold conjugates), 2) nitrocellulose membrane strip ine mabhendi maviri ekuedzwa (Pv nePf mabhendi) uye bhendi rekutonga (C band).Pv bhendi rakafanoputirwa nerimwe mbeva rinorwisa Pv-LDH masoja ekudzivirira chirwere chePv, bhendi rePf rakaputirwa nepolyclonal anti-pHRP-II masoja ekudzivirira chirwere kuti aone utachiona hwePf, uye C bhendi rakaputirwa. nembudzi anti-mbeva IgG.
Munguva yekuedzwa, huwandu hwakakwana hwemuenzaniso weropa unopihwa mumuenzaniso tsime (S) yekaseti yekuedzwa, lysis buffer inowedzerwa kune buffer tsime (B).Iyo buffer ine muchetura unoburitsa masero matsvuku eropa uye unoburitsa maantigen akasiyana siyana, ayo anofamba necapillary action achiyambuka mutsara wakabatwa mukaseti.Pv-LDH kana iripo mumuenzaniso inosungirira kune Pv-LDH-goridhe conjugates.Iyo immunocomplex inozobatwa pa membrane ne pre-coated anti-Pv-LDH antibody, ichigadzira burgundy yeruvara Pv bhendi, ichiratidza Pv yakanaka bvunzo mhedzisiro.Neimwe nzira, pHRP-II kana iripo mumuenzaniso inosunga kune pHRP-II-goridhe conjugates.Iyo immunocomplex inozobatwa pa membrane ne pre-coated anti-pHRP-II masoja ekudzivirira chirwere, achigadzira burgundy colored Pf bhendi, zvichiratidza mhedzisiro yePf yakanaka.Kusavapo kwechero mabhendi ebvunzo kunoratidza mhedzisiro yakaipa.Iyo bvunzo ine yemukati kudzora (C bhendi) iyo inofanirwa kuratidza burgundy ruvara bhendi ye immunocomplex yembudzi anti- mbeva IgG / mbeva IgG (anti-Pv-LDH uye anti-pHRPII) - goridhe conjugates zvisinei nekukura kwemavara pane chero. yemabhendi ebvunzo.Zvikasadaro, mhedzisiro yebvunzo haishande uye mufananidzo unofanirwa kuongororwa nemumwe mudziyo.
