Tsanangudzo yakadzama
Kuonekwa kwehwai pox virus antibody inoumbwa ne microplate pre-yakaputirwa nehwai pox nuclear protein antigen, enzyme markers uye mamwe anotsigira reagents, uye musimboti we enzyme-yakabatana immunoassay (ELISA) inoshandiswa kuona makwai pox antibody gwai serum sample.Munguva yekuyedza, iyo seramu yekudzora uye sampu inowongororwa inowedzerwa kune microplate ndiro, uye kana sampu ine makwai pox antibody mushure mekuiswa, inosungirirwa kune antigen pane microplate plate, uye zvimwe zvikamu zvisina kusungwa. ichabviswa mushure mekugeza;Wobva wawedzera enzyme marker kuti unyatsosunga kune antigen-antibody complex pane microplate plate;Iwo ma enzyme asina kusungwa ma marker akabva abviswa nekugezeswa, uye TMB substrate solution yakawedzerwa kumatsime, uye chigadzirwa chebhuruu chakaumbwa nekuita kwema microplate conjugates, uye kudzika kwemavara kwakanyatso kuwirirana nehuwandu hwemasoja ekudzivirira chirwere arimo. muenzaniso.Mushure mokunge mhinduro yekugumisa yakawedzerwa kugumisa maitiro, chigadzirwa chakashanduka chero;Iko kukosha kwekutora mukuita kwega kwega kunotemerwa nemuverengi wemicroplate paavelength ye450 nm kuona kana sampuli ine ma antibodies ehwai pox.