PFUPISO UYE TSANANGURO MUYEdzo
Tuberculosis chirwere chisingaperi, chinotapuriranwa chinokonzerwa zvikuru neM. TB hominis (Koch's bacillus), dzimwe nguva neM. TB bovis.Mapapu ndiwo anonyanya kunangwa, asi chero nhengo inogona kutapukirwa.
Ngozi yekutapukirwa neTB yakadzikira zvakanyanya muzana remakore rechi20.Zvisinei, kubuda kwenguva pfupi yapfuura kwemarudzi asingaurayiwi nemishonga1, kunyanya pakati pevarwere vane AIDS2, kwaita kuti vanhu vafarire TB.Huwandu hwehutachiona hwakataurwa hunosvika mamirioni masere ezviitiko pagore nekufa kwemamiriyoni matatu pagore.Huwandu hwevanhu vafa hwakapfuura 50% mune dzimwe nyika dzemuAfrica dzine huwandu hweHIV.
Kufungirwa kwekiriniki kwekutanga uye zvakawanikwa neradiographic, nekuzotevera kusimbiswa kwerabhoritari kuburikidza nekuongorora sputum uye tsika ndiyo nzira yechinyakare (s) mukuongororwa kweiyo inoshanda TB5,6.Zvisinei, nzira idzi dzinenge dzisina kunzwa kana kuti dzinotora nguva, kunyanya hadzina kukodzera kuvarwere vasingakwanisi kubudisa sputum yakakwana, smear-negative, kana kuti vanofungidzirwa kuti vane extra-pulmonary TB.
Iyo TB IgG/IgM Combo Rapid Test inogadzirwa kuti ibvise zvipingaidzo izvi.Muedzo unoona IgM neIgG anti-M.TB muserum, plasm, kana ropa rose mumaminitsi gumi nemashanu.Mhedzisiro yakanaka yeIgM inoratidza hutachiona hweM.TB hutsva, nepo mhinduro yeIgG inoratidza hutachiona hwekare kana husingaperi.Ichishandisa M.TB chaiyo maantigen, inoonawo IgM anti-M.TB muvarwere vanobaiwa neBCG.Mukuwedzera, muedzo unogona kuva
inoitwa nevashandi vasina kudzidziswa kana hunyanzvi hushoma vasina midziyo yemurabhoritari inorema.
PRINCIPLE
Iyo TB IgG/IgM Rapid Test ndeye lateral kuyerera chromatographic immunoassay.Iyo bvunzo kaseti ine: 1) burgundy yeruvara conjugate pad ine M.TB maantigen akabatanidzwa ne colloid goridhe (M.TB conjugates) uye tsuro IgG-goridhe conjugates, 2) nitrocellulose membrane strip ine maviri ebvunzo mabhendi (M uye G mabhendi. ) uye bhendi rekutonga (C band).Iyo M bhendi yakavharwa kare ne monoclonal anti-human IgM yekuona IgM anti- M.TB, G bhendi rakafanoiswa reagents kuti rionekwe IgG anti-M.TB, uye C band iri pre. -yakaputirwa nembudzi anti-tsuro IgG.
Kana vhoriyamu yakakwana yemuenzaniso webvunzo yaiswa mumuenzaniso tsime rekaseti, mufananidzo wacho unotama necapillary chiito kuyambuka kaseti.IgM anti-M.TB kana iripo mumuenzaniso inosunga kune M.TB conjugates.Iyo immunocomplex inozobatwa pa membrane ne pre-coated anti-human IgM antibody, ichigadzira burgundy ine ruvara rweM bhendi, inoratidza M.TB IgM yakanaka bvunzo mhedzisiro.IgG anti-M.TB, kana iripo mumuenzaniso, inosunga kune M.TB conjugates.Iyo immunocomplex inozotorwa nema pre-coated reagents pa membrane, ichigadzira burgundy yemavara G bhendi, ichiratidza M.TB IgG yakanaka bvunzo mhinduro.Kusavapo kwechero mabhendi ebvunzo (M naG) kunoratidza mhedzisiro yakaipa.Iyo bvunzo ine yemukati kudzora (C bhendi) iyo inofanirwa kuratidza burgundy yemavara bhendi ye immunocomplex yembudzi anti rabbit IgG/tsuro IgG-goridhe conjugate zvisinei nekukura kwemavara pane chero maT bhendi.Zvikasadaro, mhedzisiro yebvunzo haishande uye mufananidzo unofanirwa kuongororwa nemumwe mudziyo.
