Tsanangudzo yakadzama
Nzira yekuongorora
Kune nzira nhatu huru dzekuongorora dze toxoplasmosis: pathogenic diagnostic, immunological diagnosis uye molecular diagnosis.Pathogenic kuongororwa kunonyanya kusanganisira histological diagnosis, mhuka inoculation uye kuzviparadzanisa nevamwe, uye sero tsika.Iyo yakajairika serological diagnostic nzira dzinosanganisira dhayi bvunzo, isina kunanga hemagglutination bvunzo, isina kunanga immunofluorescence antibody bvunzo uye enzyme yakabatana immunosorbent assay.Molecular kuongororwa kunosanganisira PCR tekinoroji uye nucleic acid hybridization tekinoroji.
Kuongororwa kwemuviri waamai vane pamuviri kunosanganisira kuongororwa kunonzi TORCH.TORCH musanganiswa webhii rekutanga rezita rechirungu rezvirwere zvakawanda.Vara T rinomirira Toxoplasma gondii.(Mamwe mavara anomiririra syphilis, rubella virus, cytomegalovirus uye herpes simplex virus zvichiteerana.)
Tarisa musimboti
Pathogen kuongorora
1. Kuongororwa kwemicroscopic kweropa remurwere, mwongo kana cerebrospinal fluid, pleural and ascites, sputum, bronchoalveolar lavage fluid, aqueous humor, amniotic fluid, nezvimwewo zvekuzora, kana lymph nodes, tsandanyama, chiropa, placenta uye mamwe matishu mhenyu. zvikamu, zveReich kana Ji staining microscopic bvunzo inogona kuwana trophozoites kana cysts, asi chiyero chakanaka hachina kukwirira.Inogona zvakare kushandiswa kune yakananga immunofluorescence kuona Toxoplasma gondii mumatishu.
2. Kuiswa jekiseni remhuka kana kuti tissue culture Tora mvura yemuviri kana kuti tissue suspension kuti iongororwe woipinza mudumbu rembeva.Utachiona hunogona kuitika uye zvirwere zvinogona kuwanikwa.Kana chizvarwa chekutanga cheinoculation chisina kunaka, chinofanira kupfuudzwa neupofu katatu.Kana yematishu tsika (tsoko itsvo kana nguruve itsvo masero) kuparadzanisa uye kuona Toxoplasma gondii.
3. DNA hybridization tekinoroji Nyanzvi dzemudzimba dzakashandisa 32P dzakanyorwa probes dzine chaiwo maDNA sequences eToxoplasma gondii kekutanga kuita mamolecular hybridization nemasero kana matissue DNA muropa revarwere, uye zvakaratidza kuti mabhandi ekusanganisa kana makwapa aive maitiro akanaka.Zvose zvakananga uye sensitivity zvaive zvakakwirira.Pamusoro pezvo, polymerase chain reaction (PCR) yakavambwawo kuChina kuti iongorore chirwere ichi, uye kana ichienzaniswa neprobe hybridization, jekiseni rekudzivirira mhuka uye nzira dzekuongorora zvirwere, inoratidza kuti yakanyatsojeka, inonzwisisika uye inokurumidza.
Immunological kuongorora
1. Maantigen anoshandiswa kuona masoja ekudzivirira chirwere anonyanya kusanganisira tachyzoite soluble antigen (cytoplasmic antigen) uye membrane antigen.Antibody yepakutanga yakaonekwa kare (yakaonekwa nekusvibisa bvunzo uye isina kunanga immunofluorescence bvunzo), nepo iyo yekupedzisira yakaonekwa gare gare (yakaonekwa neindirect hemagglutination test, nezvimwewo).Panguva imwecheteyo, nzira dzakawanda dzekuona dzinogona kuita basa rekuwedzera uye kuvandudza mwero wekuona.Nekuti Toxoplasma gondii inogona kuvapo mumasero emunhu kwenguva yakareba, zvakaoma kusiyanisa utachiona hwazvino kana hutachiona hwekare nekuona masoja ekudzivirira chirwere.Inogona kutongwa zvichienderana neiyo antibody titer uye shanduko yayo ine simba.
2. Detection antigen inoshandiswa kuona zvirwere (tachyzoites kana cysts) mumasero anotakura, metabolites kana lysis zvigadzirwa (inotenderera maantigens) mu serum uye mvura yemuviri nenzira dzeimmunological.Iyo inzira yakavimbika yekuongororwa kwekutanga uye yechokwadi kuongororwa.Nyanzvi dzekumba nekune dzimwe nyika dzakagadzira McAb ELISA uye sandwich ELISA pakati peMcAb nemultiantibody kuti ione kutenderera kweantigen muserum yevarwere vakaoma, nekunzwa kwe0.4 μ G/ml yeantigen.
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Uncut Sheet Rapid Test Manufacturing process
